mitochondrial function analyses Search Results


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Revvity operetta
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Broad Institute Inc gsea software
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Strathkelvin Instruments Limited mitochondrial function analyzer mt 200
Mitochondrial Function Analyzer Mt 200, supplied by Strathkelvin Instruments Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Oroboros Instruments high resolution respirometry
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Strathkelvin Instruments Limited mitochondrial function analyzer mt200
Effects of LR solution, HES solution, and LR solution with the addition of NAC (30 mg/kg) on <t>mitochondrial</t> function. (A) RCR of heart; (D) RCR of intestine; (C) RCR of kidney; (B) RCR of liver. RCR, respiratory control rate; LR, Lactated Ringer’s; HES, Hetastarch; NAC, LR solution with the addition of NAC (30 mg/kg). Data presented as mean ± standard deviation ( n = 8/group). ∧∧∧ P < 0.001, Sea-Shock group versus baseline; † P < 0.05, ††† P < 0.001, LR group or HES group versus Sea-Shock group; ## P < 0.01, ### P < 0.001, LR group versus NAC group; ** P < 0.01, *** P < 0.001, HES group versus NAC group.
Mitochondrial Function Analyzer Mt200, supplied by Strathkelvin Instruments Limited, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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Promega celltiter 96 aqueous one solution cell proliferation assay
Effects of LR solution, HES solution, and LR solution with the addition of NAC (30 mg/kg) on <t>mitochondrial</t> function. (A) RCR of heart; (D) RCR of intestine; (C) RCR of kidney; (B) RCR of liver. RCR, respiratory control rate; LR, Lactated Ringer’s; HES, Hetastarch; NAC, LR solution with the addition of NAC (30 mg/kg). Data presented as mean ± standard deviation ( n = 8/group). ∧∧∧ P < 0.001, Sea-Shock group versus baseline; † P < 0.05, ††† P < 0.001, LR group or HES group versus Sea-Shock group; ## P < 0.01, ### P < 0.001, LR group versus NAC group; ** P < 0.01, *** P < 0.001, HES group versus NAC group.
Celltiter 96 Aqueous One Solution Cell Proliferation Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Beyotime mptp assay kit
Functional measurements of mitochondrial activity in <t>rescued</t> <t>tenocytes.</t> A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in <t>mPTP</t> opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001
Mptp Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bucher Biotec AG seahorse bioscience xf24 extracellular flux analyzer
Functional measurements of mitochondrial activity in <t>rescued</t> <t>tenocytes.</t> A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in <t>mPTP</t> opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001
Seahorse Bioscience Xf24 Extracellular Flux Analyzer, supplied by Bucher Biotec AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MetaMorph Inc tracking function
Functional measurements of mitochondrial activity in <t>rescued</t> <t>tenocytes.</t> A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in <t>mPTP</t> opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001
Tracking Function, supplied by MetaMorph Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MathWorks Inc connectivity toolbox v21a conn
Functional measurements of mitochondrial activity in <t>rescued</t> <t>tenocytes.</t> A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in <t>mPTP</t> opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001
Connectivity Toolbox V21a Conn, supplied by MathWorks Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Dojindo Labs cck 8 assay kit
Functional measurements of mitochondrial activity in <t>rescued</t> <t>tenocytes.</t> A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in <t>mPTP</t> opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001
Cck 8 Assay Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Effects of LR solution, HES solution, and LR solution with the addition of NAC (30 mg/kg) on mitochondrial function. (A) RCR of heart; (D) RCR of intestine; (C) RCR of kidney; (B) RCR of liver. RCR, respiratory control rate; LR, Lactated Ringer’s; HES, Hetastarch; NAC, LR solution with the addition of NAC (30 mg/kg). Data presented as mean ± standard deviation ( n = 8/group). ∧∧∧ P < 0.001, Sea-Shock group versus baseline; † P < 0.05, ††† P < 0.001, LR group or HES group versus Sea-Shock group; ## P < 0.01, ### P < 0.001, LR group versus NAC group; ** P < 0.01, *** P < 0.001, HES group versus NAC group.

Journal: Frontiers in Physiology

Article Title: N-Acetyl-L-Cysteine Protects Organ Function After Hemorrhagic Shock Combined With Seawater Immersion in Rats by Correcting Coagulopathy and Acidosis

doi: 10.3389/fphys.2022.831514

Figure Lengend Snippet: Effects of LR solution, HES solution, and LR solution with the addition of NAC (30 mg/kg) on mitochondrial function. (A) RCR of heart; (D) RCR of intestine; (C) RCR of kidney; (B) RCR of liver. RCR, respiratory control rate; LR, Lactated Ringer’s; HES, Hetastarch; NAC, LR solution with the addition of NAC (30 mg/kg). Data presented as mean ± standard deviation ( n = 8/group). ∧∧∧ P < 0.001, Sea-Shock group versus baseline; † P < 0.05, ††† P < 0.001, LR group or HES group versus Sea-Shock group; ## P < 0.01, ### P < 0.001, LR group versus NAC group; ** P < 0.01, *** P < 0.001, HES group versus NAC group.

Article Snippet: The rate of oxygen consumption was determined by a mitochondrial function analyzer (MT200; Strathkelvin) ( n = 8/group).

Techniques: Control, Standard Deviation

Functional measurements of mitochondrial activity in rescued tenocytes. A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in mPTP opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001

Journal: Stem Cell Research & Therapy

Article Title: Mitochondrial transfer from bone mesenchymal stem cells protects against tendinopathy both in vitro and in vivo

doi: 10.1186/s13287-023-03329-0

Figure Lengend Snippet: Functional measurements of mitochondrial activity in rescued tenocytes. A ΔΨm was determined by confocal microscopy using JC-1 dye ( n = 6). JC-1 aggregates show red and JC-1 monomers green fluorescence. The nuclei were stained with Hoechst 33,342 (blue). Scale bar: 400 × , 25 µm. B Fluorescence quantitative analysis of ΔΨm for Fig. 2A. C Representative graphs illustrate the decrease in mPTP opening after MSC treatment. Quantitative mPTP in tenocytes is represented by RFI ( n = 3). D Quantitative analysis of ATP using the ATP determination kit ( n = 6). E OCR were determined by Seahorse XFe 24 Analyzer and normalized to protein content ( n = 6). F Representative western blots of total protein expression of Drp1 and Mfn2 ( n = 3). G Histogram analysis showing the relative protein levels for Fig. 2F. Data represent mean ± SD. Statistical significance of the differences among groups was determined by ANOVA (Bonferroni or LSD method). * P < 0.05, ** P < 0.01, *** P < 0.001

Article Snippet: mPTP of sorted tenocytes was analyzed using the mPTP Assay Kit (Beyotime) as previously described [ ].

Techniques: Functional Assay, Activity Assay, Confocal Microscopy, Fluorescence, Staining, Western Blot, Expressing